Cheese samples were inoculated into tryptone-peptone-glucose-yeast extract toxin-production media, incubated for seven days, and then assayed for toxin comparing the amplified ELISA and the mouse bioassay. The culture supernatants were tested for type A, B, E, and F botulinal toxins. The amplified ELISA system indicated the product contained type A botulinal toxin-producing organisms that produced greater than 1,000 MLD/ml in the culture. No cross-reactivity was noted between the toxic cultures and the other ELISA test types B, E, or F. The sensitivity of this one day ELISA test was ~10 MLD/ml of culture fluid compared to the mouse bioassay test (1 MLD/ml). The advantages of this test are that the toxin type and approximate lethal dose are rapidly determined (one day) compared to longer analytical time (3-5 days) and the necessity of using animals by the classical procedure. The procedure is also useful for the identification of toxin-producing botulinal isolates obtained from agar plates.