A simple, rapid procedure to generate an oxygen-reduced atmosphere suitable for growing Campylobacter jejuni was investigated. A modified AnaeroGenTM System (MAS), consisting of a single AnaeroGenTM anaerobic sachet AN35 (Oxoid Unipath Ltd., Basingstoke, UK) activated in a 9-liter anaerobic jar (BBL, Cockeysville, MD), was evaluated and compared with the conventional gassed jar system described in the 8th edition of the U.S. Food and Drug Administration³s Bacteriological Analytical Manual (BAM). Enriched cultures of C. Jejuni in artificially contaminated raw milk, raw oyster, crab meat, or mushroom were streaked in duplicate onto four different selective isolation agars for simultaneous incubation in MAS and in the BAM system. No significant differences (p > 0.05) in recovery rates of C. Jejuni were observed for the two systems. The type of isolation agar used did not affect these recovery rates. The quality of growth of C. Jejuni at 24, 48, or 72 h was similar in both systems. MAS successfully reduced atmospheric oxygen to a level suitable for the growth of C. Jejuni. It was simple and rapid compared to the BAM system, and cost-effective compared to the Oxoid CampyGenTM system specifically designed for the growth of Campylobacter spp.